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Registro Completo |
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Biblioteca(s): |
Embrapa Roraima. |
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Data corrente: |
02/12/2016 |
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Data da última atualização: |
22/10/2019 |
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Autoria: |
COELHO, L. C. |
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Título: |
Qualidade pós-colheita de vagens frescas de feijão-caupi (Vigna unguiculata (L.) Walp) CV. tumucumaque cultivados sob diferentes doses de adubação com fósforo e zinco. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
UERR, 2016. |
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Idioma: |
Português |
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Notas: |
Monografia (Graduação) - Universidade Estadual de Roraima, 2016. Orientadora Dra. Maria Fernanda Berlingieri Durigan. |
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Palavras-Chave: |
Feijão vagem; Hortaliças. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00562nam a2200133 a 4500
001 2057748
005 2019-10-22
008 2016 bl uuuu m 00u1 u #d
100 1 $aCOELHO, L. C.
245 $aQualidade pós-colheita de vagens frescas de feijão-caupi (Vigna unguiculata (L.) Walp) CV. tumucumaque cultivados sob diferentes doses de adubação com fósforo e zinco.
260 $aUERR$c2016
500 $aMonografia (Graduação) - Universidade Estadual de Roraima, 2016. Orientadora Dra. Maria Fernanda Berlingieri Durigan.
653 $aFeijão vagem
653 $aHortaliças
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Registro original: |
Embrapa Roraima (CPAF-RR) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
22/12/2015 |
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Data da última atualização: |
22/12/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
MACHADO-DE-ÁVILA, R. A.; VELLOSO, M.; OLIVEIRA, D.; STRANSKY, S.; FLOR-SÁ, A.; SCHNEIDER, F. S.; NESHICH, G.; CHÁVEZ-OLÓRTEGUI, C. |
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Afiliação: |
RICARDO ANDREZ MACHADO-DE-ÁVILA, Universidade do Extremo Sul Catarinense, Criciúma-SC; MARIANA VELLOSO, UFMG; DAYSIANE OLIVEIRA, UFMG; STEPHANIE STRANSKY, UFMG; ANA FLOR-SÁ, UFMG; FRANCISCO S. SCHNEIDER, UFMG; GORAN NESHICH, CNPTIA; CARLOS CHÁVEZ-OLÓRTEGUI, Universidade do Extremo Sul Catarinense, Criciúma-SC. |
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Título: |
Induction of neutralizing antibodies against mutalysin-II from Lachesis muta muta Snake Venom Elicited by a conformational B-cell epitope predicted by Blue Star Sting data base. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Immunome Research, v. 11, n. 1, Mar. 2015. |
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Páginas: |
Não paginado. |
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DOI: |
http://dx.doi.org/10.4172/1745-7580.1000083 |
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Idioma: |
Inglês |
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Conteúdo: |
Mutalysin-II, from Lachesis muta muta snake venom, is an endopeptidase with hemorrhagic activity. To identify a conformational epitope we used Blue Star Sting, the latest version of the web based Sting Millenium Suite, as alternative computational analysis tool to select and design peptides. Pre-selected cut-off values of the accessibility and hydrophilicity parameters were used to select amino acid residues as potential conformational epitopes. A peptide (P117-Y116-C115-Q194-C195-L197-N198-K199-P200-Y5-L48) was manually drawn on Swiss-PDB-Viewer package and synthesized by Fmoc-synthesis. Immunization of rabbits with this peptide induced antibodies that recognized Mutalysin-II and protected against the hemorrhagic factors present in Lachesis venom. The Sting Millennium Suite was able to predict conformational epitopes in this class of proteins. Three amino acids (K199, Y5 and L48) were identified as essential in the interaction between the peptide and the neutralizing antibodies. |
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Palavras-Chave: |
Conformational epitope prediction; Peptídeos; Sting millennium. |
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Thesagro: |
Veneno. |
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Thesaurus NAL: |
Antivenoms; Epitopes; Lachesis muta; Synthetic peptides. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136041/1/induction-Avila.pdf
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Marc: |
LEADER 02032naa a2200325 a 4500
001 2032210
005 2015-12-22
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4172/1745-7580.1000083$2DOI
100 1 $aMACHADO-DE-ÁVILA, R. A.
245 $aInduction of neutralizing antibodies against mutalysin-II from Lachesis muta muta Snake Venom Elicited by a conformational B-cell epitope predicted by Blue Star Sting data base.$h[electronic resource]
260 $c2015
300 $aNão paginado.
520 $aMutalysin-II, from Lachesis muta muta snake venom, is an endopeptidase with hemorrhagic activity. To identify a conformational epitope we used Blue Star Sting, the latest version of the web based Sting Millenium Suite, as alternative computational analysis tool to select and design peptides. Pre-selected cut-off values of the accessibility and hydrophilicity parameters were used to select amino acid residues as potential conformational epitopes. A peptide (P117-Y116-C115-Q194-C195-L197-N198-K199-P200-Y5-L48) was manually drawn on Swiss-PDB-Viewer package and synthesized by Fmoc-synthesis. Immunization of rabbits with this peptide induced antibodies that recognized Mutalysin-II and protected against the hemorrhagic factors present in Lachesis venom. The Sting Millennium Suite was able to predict conformational epitopes in this class of proteins. Three amino acids (K199, Y5 and L48) were identified as essential in the interaction between the peptide and the neutralizing antibodies.
650 $aAntivenoms
650 $aEpitopes
650 $aLachesis muta
650 $aSynthetic peptides
650 $aVeneno
653 $aConformational epitope prediction
653 $aPeptídeos
653 $aSting millennium
700 1 $aVELLOSO, M.
700 1 $aOLIVEIRA, D.
700 1 $aSTRANSKY, S.
700 1 $aFLOR-SÁ, A.
700 1 $aSCHNEIDER, F. S.
700 1 $aNESHICH, G.
700 1 $aCHÁVEZ-OLÓRTEGUI, C.
773 $tImmunome Research$gv. 11, n. 1, Mar. 2015.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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